Buy Kisspeptin-10 in Canada from Anglo Peptides, a Canadian research-grade supplier of the reproductive-endocrine peptide Kisspeptin-10. Every vial is produced in a cGMP-compliant facility, independently verified to ≥99% purity by third-party HPLC, and lyophilised for stability. Orders ship same-day in cold-pack packaging, arrive within 1 to 3 business days across Canada, and include a batch-specific certificate of analysis. The summary below reflects the published research literature and is provided for laboratory research context only.
What Is Kisspeptin-10?
Kisspeptin-10 is the bioactive C-terminal decapeptide fragment of the kisspeptin family, a group of RF-amide neuropeptides produced from the KISS1 gene. The KISS1 precursor is processed into kisspeptin-54 and shorter fragments including kisspeptin-10, all of which share a C-terminal Arg-Phe-amide motif required to activate their receptor. Kisspeptin-10 is an agonist ligand of KISS1R, also known as GPR54. The human sequence is Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH2, with CAS number 374675-21-5, molecular formula C63H83N17O14 and a molecular weight of roughly 1302 g/mol. Researchers should note that the rodent sequence differs at the C-terminus and is a distinct compound, so the species form should always be confirmed against the certificate of analysis.
Mechanism of Action
Kisspeptin-10 binds the KISS1R/GPR54 receptor on gonadotropin-releasing-hormone (GnRH) neurons in the hypothalamus. Receptor activation, through Gq and phospholipase-C signalling, stimulates these neurons to secrete GnRH into the hypophyseal portal system.[1] GnRH in turn acts on the anterior pituitary to trigger release of the gonadotropins luteinising hormone (LH) and, to a lesser degree, follicle-stimulating hormone (FSH). This positions kisspeptin and KISS1R signalling as a key upstream gatekeeper of the hypothalamic-pituitary-gonadal (HPG) axis, integrating sex-steroid feedback and metabolic signals into the control of reproduction. Human genetic studies established the importance of this pathway: loss-of-function of GPR54/KISS1R causes a failure of pubertal maturation, while activating mutations are associated with precocious puberty.[2][3]
Research Applications
Kisspeptin-10 is a central tool compound in reproductive endocrinology, with both preclinical and investigational clinical research behind it.
Receptor and puberty biology. Kisspeptins were identified as the natural ligands of the orphan receptor GPR54,[1] and independent human-genetics work established the kisspeptin-GPR54 system as essential for puberty and reproduction.[2][3]
HPG-axis stimulation in humans. Administration of kisspeptin raises circulating LH (and FSH and testosterone) in healthy men, and kisspeptin-10 acutely stimulates LH and increases LH pulse frequency.[4][6] In women, the gonadotropin response is strongly dependent on menstrual-cycle phase, being most potent in the preovulatory phase.[5]
Research uses. It is used to probe GnRH-pulse-generator function and in studies of fertility, ovulation triggering, pubertal timing and the metabolic regulation of reproduction, underpinned by rodent, primate and ovine work. Kisspeptin-10 is not an approved therapeutic and is supplied for laboratory research only.
Stability, Solubility and Handling
Anglo Peptides supplies Kisspeptin-10 as a lyophilised powder. For research it is dissolved in sterile or bacteriostatic water or an appropriate buffer, swirling gently rather than vortexing. Store the lyophilised powder desiccated and protected from light, with long-term storage at -20°C; keep reconstituted solution refrigerated and use within a limited window, aliquoting to avoid repeated freeze-thaw cycles. As the sequence contains tryptophan, minimise air exposure to limit oxidation. These notes apply to in-vitro and animal research only.
Product Specifications
- Compound: Kisspeptin-10 (human), KISS1R/GPR54 agonist decapeptide
- Sequence: Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH2
- CAS: 374675-21-5 (human sequence)
- Molecular formula: C63H83N17O14
- Molecular weight: ~1302 g/mol
- Purity: ≥99% by HPLC
- Size: 5 mg per vial
- Form: Lyophilised powder
- Testing: Independent third-party HPLC and mass spectrometry
- Documentation: Batch-specific certificate of analysis
- Origin: Made in Canada
Quality, Testing and Canadian Sourcing
Every batch of Anglo Peptides Kisspeptin-10 is independently tested to ≥99% purity with a lot-specific certificate of analysis. Producing and finishing vials in Canada lets us verify the full labelled milligram content and ship quickly without customs delays. Read more on our Canadian sourcing page. Researchers studying the growth-hormone and reproductive axes often work with related secretagogues such as Sermorelin and Ipamorelin.
Kisspeptin and the HPG Axis in Research
Kisspeptin signalling occupies a central position in the neuroendocrine control of reproduction, which is why kisspeptin-10 has become a widely used probe in the field. The neurons that express kisspeptin, particularly the population in the arcuate nucleus often described together with neurokinin B and dynorphin, are thought to form part of the pulse generator that drives episodic GnRH release. By administering kisspeptin-10 and measuring the downstream luteinising-hormone response, investigators can interrogate the integrity and responsiveness of this axis in a controlled way. This makes the peptide valuable across reproductive-endocrinology research, from studies of pubertal timing and the metabolic gating of fertility to investigations of conditions in which the axis is suppressed. Its position upstream of GnRH also makes it a useful contrast to the growth-hormone secretagogues, which act on a separate hypothalamic-pituitary pathway.
Considerations for Research Use
The single most important design point for kisspeptin-10 is sequence identity: the human and rodent decapeptides differ at the C-terminus and are distinct compounds, so the species form must match the model and be confirmed against the certificate of analysis. A second consideration, drawn from the human literature, is that the gonadotropin response in females is strongly dependent on cycle phase, so reproductive status must be accounted for in any design measuring downstream hormones. Because the tryptophan residue in the sequence is oxidation-sensitive, solutions should be protected from air and light. As with any peptide, confirmation of identity by mass spectrometry and verification of purity for each lot are essential, since an under-dosed or degraded preparation will distort the hormonal readout. Every Anglo Peptides batch ships with third-party HPLC verification and a lot-specific certificate of analysis.
Reconstitution, Storage and Handling in Detail
For laboratory use, the lyophilised powder is typically brought to room temperature before opening to avoid condensation, then reconstituted by adding sterile or bacteriostatic water slowly down the inside wall of the vial rather than directly onto the powder, and swirled gently rather than shaken to limit foaming and shear stress. The choice of diluent depends on the study: sterile water is common for single-session work, while bacteriostatic water, which contains a small amount of benzyl alcohol, is often preferred when a solution will be drawn from over several days. Once reconstituted, the solution is kept refrigerated at 2 to 8 degrees Celsius, protected from light, and used within a defined window, with aliquoting into single-use portions the most reliable way to avoid the repeated freeze-thaw cycles that degrade peptides. The lyophilised powder itself is far more stable than any solution and is best stored frozen, sealed and desiccated, for long-term holding. These are general research-handling conventions; the lot-specific certificate of analysis and its storage guidance should always take precedence for a given batch.
Frequently Asked Questions
What is Kisspeptin-10?
How does Kisspeptin-10 work?
Does the species sequence matter?
Is Kisspeptin-10 approved for human use?
Why does Kisspeptin-10 raise LH?
Does it matter whether the sequence is human or rodent?
Why is the female response cycle-dependent?
How should Kisspeptin-10 be handled?
References
- Kotani M, Detheux M, Vandenbogaerde A, et al. The metastasis suppressor gene KiSS-1 encodes kisspeptins, the natural ligands of the orphan G protein-coupled receptor GPR54. J Biol Chem. 2001;276(37):34631-34636. PubMed
- de Roux N, Genin E, Carel JC, et al. Hypogonadotropic hypogonadism due to loss of function of the KiSS1-derived peptide receptor GPR54. Proc Natl Acad Sci USA. 2003;100(19):10972-10976. PubMed
- Seminara SB, Messager S, Chatzidaki EE, et al. The GPR54 gene as a regulator of puberty. N Engl J Med. 2003;349(17):1614-1627. PubMed
- Dhillo WS, Chaudhri OB, Patterson M, et al. Kisspeptin-54 stimulates the hypothalamic-pituitary-gonadal axis in human males. J Clin Endocrinol Metab. 2005;90(12):6609-6615. PubMed
- Dhillo WS, Chaudhri OB, Thompson EL, et al. Kisspeptin-54 stimulates gonadotropin release most potently during the preovulatory phase of the menstrual cycle in women. J Clin Endocrinol Metab. 2007;92(10):3958-3966. PubMed
- George JT, Veldhuis JD, Roseweir AK, et al. Kisspeptin-10 is a potent stimulator of LH and increases pulse frequency in men. J Clin Endocrinol Metab. 2011;96(8):E1228-E1236. PubMed
Research use only. Kisspeptin-10 supplied by Anglo Peptides is intended exclusively for licensed laboratory and academic research. It is not a drug, dietary supplement, or medical product, and is not for human consumption, diagnosis, or treatment.
Researchers who buy Kisspeptin from Anglo Peptides receive a vial verified to ≥99% purity, batch-tested by Janoshik Labs, and shipped from within Canada. Choose the 5mg size above.
Every order is filled and finished in Canada, then dispatched domestically with tracking. Vials ship discreetly and clear in days, not weeks — no customs holds and no border delays that stall a study.
Each batch is independently verified by Janoshik Labs before it is listed, so identity and purity are documented rather than assumed. A certificate of analysis is available on request, and every vial links back to the lot it came from.
For handling, store unopened vials frozen and reconstitute with bacteriostatic water only when a study calls for it. Keep the reconstituted solution refrigerated, avoid repeated freeze-thaw cycles, and label each vial with its reconstitution date.
Omar A. –
Anglo is one of the few Canadian sources with Kisspeptin-10. Shipped fast, came well packaged, reconstituted clean. Good quality overall.
Jia Wu –
Good product. Arrived in two days, vial sealed properly. Dissolved cleanly with no residue. Happy with the quality and will order again from Anglo.
Chloe G. –
Kisspeptin-10 from Anglo Peptides is one of the niche products I was having trouble finding from a Canadian manufacturer. Quality is excellent and the Janoshik COA confirms it.
Ethan P. –
Good quality and fast Xpresspost to London, Ontario. COA is detailed. Reconstituted cleanly in BAC water. Appreciate that Anglo carries these harder-to-find compounds domestically.